4AD8
Crystal structure of a deletion mutant of Deinococcus radiodurans RecN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 72.205, 62.487, 145.526 |
| Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
| Resolution | 47.031 - 3.998 |
| R-factor | 0.326 |
| Rwork | 0.325 |
| R-free | 0.34700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aby |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.694 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 4.210 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.280 | 0.760 |
| Number of reflections | 5430 | |
| <I/σ(I)> | 3.8 | 1.5 |
| Completeness [%] | 97.5 | 93.8 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM CACODYLATE TRIHYDRATE PH 6.5, 0.2 M MAGNESIUM ACETATE TETRAHYDRATE, 10% W/V PEG 8000 |
