4ACR
Crystal structure of N-glycosylated, C-terminally truncated human glypican-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.170, 168.630, 147.760 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 29.714 - 2.550 |
| R-factor | 0.253 |
| Rwork | 0.251 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.717 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 79825 | |
| <I/σ(I)> | 11.6 | 2.4 |
| Completeness [%] | 98.9 | 94.9 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 12-14% PEG 6000, 0.1 M TRIS-HCL, 0.2 M CACL2, PH 8.0 |
