4ACL
3D Structure of DotU from Francisella novicida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-26 |
| Detector | RIGAKU R-AXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.309, 85.314, 66.989 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 65.430 - 2.490 |
| R-factor | 0.22117 |
| Rwork | 0.218 |
| R-free | 0.27867 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.649 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.990 | 2.600 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Rmerge | 0.120 | 0.500 |
| Number of reflections | 15228 | |
| <I/σ(I)> | 10.8 | 4.1 |
| Completeness [%] | 98.3 | 93.2 |
| Redundancy | 13.2 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS, PH 8.8, 20% PEG 3350. |
