4ACK
3D Structure of DotU from Francisella novicida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.660, 85.840, 67.710 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 65.990 - 2.150 |
| R-factor | 0.19691 |
| Rwork | 0.194 |
| R-free | 0.25118 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.778 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.990 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 24116 | |
| <I/σ(I)> | 14.9 | 5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.115 M KBR, 0.1 M TRIS, PH 8.5, 20% PEG 3350 |
