4ACA

CRYSTAL STRUCTURE OF TRANSLATION ELONGATION FACTOR SELB FROM METHANOCOCCUS MARIPALUDIS, APO FORM

Replaces:  1WB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-19
Detector	MARRESEARCH
Spacegroup name	P 31 1 2
Unit cell lengths	146.860, 146.860, 297.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.971 - 3.150
R-factor	0.1743
Rwork	0.172
R-free	0.21480
Structure solution method	SAD
Starting model (for MR)	4ac9
RMSD bond length	0.007
RMSD bond angle	1.126
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.340
High resolution limit [Å]	3.150	3.150
Rmerge	0.110	1.640
Number of reflections	124004
<I/σ(I)>	15.23	1.71
Completeness [%]	99.7	99.7
Redundancy	8.7	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				10 MM KCL,20 MM TRIS/HCL (PH 7.5),40 MM MGSO4,2 MM DTT,3 MM GDP,1.8 - 2.0 M AMMONIUM SULFATE,100 MM SODIUM CITRATE (PH 6.0),1 MM DEOXYCHOLIC ACID