4ABY
Crystal structure of Deinococcus radiodurans RecN head domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.863, 61.986, 133.824 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 49.510 - 3.000 |
| R-factor | 0.2115 |
| Rwork | 0.209 |
| R-free | 0.24880 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.785 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.300 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 41994 | |
| <I/σ(I)> | 9.2 | 3.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | TRIS 0.1 M PH 7.5, 25% PEG 3350. |
