4ABU
CRYSTAL STRUCTURE OF TRANSTHYRETIN IN COMPLEX WITH LIGAND C-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.600, 42.470, 63.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.870 - 1.860 |
| R-factor | 0.20805 |
| Rwork | 0.206 |
| R-free | 0.25371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dvq |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.069 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 5.800 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 20168 | |
| <I/σ(I)> | 12.5 | 3 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | POTASSIUM CHLORIDE, POTASSIUM PHOSPHATE, AMMONIUM SULFATE, PH 7.4, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
