4A9J
N-TERMINAL BROMODOMAIN OF HUMAN BRD2 WITH N-(4-hydroxyphenyl) acetamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Collection date | 2010-06-26 |
| Detector | RIGAKU A200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.051, 55.542, 68.072 |
| Unit cell angles | 90.00, 94.88, 90.00 |
Refinement procedure
| Resolution | 67.830 - 1.900 |
| R-factor | 0.18548 |
| Rwork | 0.184 |
| R-free | 0.21828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.830 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 33482 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.1 M HEPES PH 7.0, 24-28% PEG 3350, 0.2 M (NH4)2SO4, 20 DEGREES CELSIUS. |
