4A91

Crystal structure of the glutamyl-queuosine tRNAAsp synthetase from E. coli complexed with L-glutamate

Replaces:  2ZLZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	C 2 2 21
Unit cell lengths	39.100, 115.030, 154.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.353 - 1.750
R-factor	0.1662
Rwork	0.165
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nzj
RMSD bond length	0.006
RMSD bond angle	0.981
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.800
High resolution limit [Å]	1.750	1.750
Rmerge	0.050	0.100
Number of reflections	35738
<I/σ(I)>	25.4	7
Completeness [%]	96.1	75.5
Redundancy	3.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M MG-ACETATE AND NA-CACODYLATE BUFFER (PH 5.5), 0.2 M KCL, 10% POLYETHYLENE GLYCOL 8000, AND 2 MM L-GLU.