4A8O

Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	106.190, 92.450, 141.040
Unit cell angles	90.00, 101.47, 90.00

Refinement procedure

Resolution	39.090 - 2.670
R-factor	0.2156
Rwork	0.215
R-free	0.22470
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	0.990
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.000	2.800
High resolution limit [Å]	2.670	2.670
Rmerge	0.090
Number of reflections	74265
<I/σ(I)>	17	2
Completeness [%]	98.0	94
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				12% PEG 20000, 100 MM MES (PH 6.5)