4A8J
Crystal Structure of the Elongator subcomplex Elp456
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.730, 124.890, 146.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.100 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.23050 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.130 | 0.660 |
| Number of reflections | 112115 | |
| <I/σ(I)> | 7.3 | 2.56 |
| Completeness [%] | 98.8 | 96.2 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200 MM MGCL2, 100 MM TRISHCL PH 7.5, AND 25% PEG 2K MME |
