4A7X
Crystal structure of uridylate kinase from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.136, 127.627, 93.181 |
| Unit cell angles | 90.00, 91.46, 90.00 |
Refinement procedure
| Resolution | 96.400 - 2.490 |
| R-factor | 0.2163 |
| Rwork | 0.213 |
| R-free | 0.27571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bnd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 62731 | |
| <I/σ(I)> | 21.3 | 2.3 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
