Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 158.040, 158.040, 142.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.290 - 3.001 |
| R-factor | 0.1727 |
| Rwork | 0.170 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v6m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.870 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.630 |
| Number of reflections | 39979 | |
| <I/σ(I)> | 19.96 | 2.22 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 3.23 | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2.4 M NAFORMATE, 0.1 M MES PH 6.0. DROPLETS ARE 2 MICROL PROTEIN PLUS 2 MICROL PRECIPITANT |
