4A6T
Crystal structure of the omega transaminase from Chromobacterium violaceum in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-06 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.490, 62.320, 119.520 |
| Unit cell angles | 105.44, 90.90, 104.52 |
Refinement procedure
| Resolution | 29.339 - 1.800 |
| R-factor | 0.1666 |
| Rwork | 0.165 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 210786 | |
| <I/σ(I)> | 12.7 | 3.7 |
| Completeness [%] | 93.5 | 83.6 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | DROPS OF 200 UL PROTEIN AT 12 MG/ML MIXED WITH 200 UL OF 0.1 M HEPES PH 7.5, 150-300 MM NACL AND 22.5-27.5% PEG 4000. |
