4A6O

CpGH89CBM32-4, produced by Clostridium perfringens, in complex with glcNAc-alpha-1,4-galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	113
Spacegroup name	P 21 21 2
Unit cell lengths	89.710, 49.890, 63.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.28656
Rwork	0.285
R-free	0.31772
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	1.053
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.150
Number of reflections	7240
Completeness [%]	98.7
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				CBM32-4 (20 MG/ML) IN COMPLEX WITH GLCNAC-ALPHA-1,4-GAL (AT 2 MM) CRYSTALLIZED IN 0.1 M ZNOAC, 0.1 M BICINE PH 8.0, 18% PEG 3350, 4 MM CRCL