4A6K
Crystal structure of Slm1-PH domain in complex with D-myo-Inositol-4- phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.950, 71.610, 82.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.790 - 1.800 |
| R-factor | 0.19514 |
| Rwork | 0.192 |
| R-free | 0.24957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.057 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.640 |
| Number of reflections | 80522 | |
| <I/σ(I)> | 15.2 | 2.02 |
| Completeness [%] | 94.8 | 90.3 |
| Redundancy | 4.53 | 3.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PH 6.5 |
