4A5N
Redoxregulator HypR in its reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.930, 68.030, 60.420 |
| Unit cell angles | 90.00, 97.45, 90.00 |
Refinement procedure
| Resolution | 59.910 - 1.810 |
| R-factor | 0.18544 |
| Rwork | 0.183 |
| R-free | 0.22287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hzt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.526 |
| Data reduction software | MOSFLM (7.0.4) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.900 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.080 | 0.530 |
| Number of reflections | 5528 | |
| <I/σ(I)> | 8.9 | 2.2 |
| Completeness [%] | 96.5 | 96.5 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2 UL PROTEIN (20 MM TRIS PH 7.5, 150 MM NACL, 15 MG/ML) AND 2 UL WELL SOLUTION (12% PEG 8000), CRYOPROTECTANT: 20% PEG 4000, 10% PEG 400. |
