4A5M
Redox regulator HypR in its oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 82.851, 82.851, 354.436 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.610 - 3.000 |
| R-factor | 0.20965 |
| Rwork | 0.207 |
| R-free | 0.24966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 1.090 |
| Number of reflections | 24764 | |
| <I/σ(I)> | 14.92 | 1.38 |
| Completeness [%] | 89.8 | 77.4 |
| Redundancy | 5.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 6% PEG 8000 0.4 M LI2SO4, CRYOPROTECTANT 15% PEG8000, 15% PEG 400, pH 7.5 |
