4A5K
Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.300, 82.300, 76.400 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 38.200 - 1.760 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nsu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.700 |
| Number of reflections | 49444 | |
| <I/σ(I)> | 23 | 2.2 |
| Completeness [%] | 83.0 | 40 |
| Redundancy | 6.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
