4A56
Crystal structure of the type 2 secretion system pilotin from Klebsiella Oxytoca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 230 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.507, 46.896, 109.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.840 - 1.240 |
| R-factor | 0.17406 |
| Rwork | 0.173 |
| R-free | 0.18975 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 4.000 |
| High resolution limit [Å] | 1.240 |
| Rmerge | 0.020 |
| Number of reflections | 47490 |
| <I/σ(I)> | 52 |
| Completeness [%] | 97.4 |
| Redundancy | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES PH 7.5, 75% MPD |
