4A4I

Crystal structure of the human Lin28b cold shock domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-26
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	46.181, 62.477, 77.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.590 - 1.950
R-factor	0.18367
Rwork	0.181
R-free	0.23464
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.768
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.590	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.080	0.240
Number of reflections	16277
<I/σ(I)>	9.5	4.1
Completeness [%]	96.6	94.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		2 M AMMONIUM SULFATE, 0.2 M NACL, 0.1 M MES PH 6.5