4A3Y
Crystal structure of Raucaffricine glucosidase from ajmaline biosynthesis pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-18 |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.774, 127.329, 215.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.150 |
| R-factor | 0.16593 |
| Rwork | 0.165 |
| R-free | 0.20355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jf7 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 76299 | |
| <I/σ(I)> | 24.6 | 4.4 |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 15.3 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.01 TO 0.3 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, PH 4.5 TO 5.0, 9 TO 12% (W/V) PEG 4000 |
