4A3W
Crystal structure of Archaemetzincin (AmzA) from Archaeoglobus fulgidus at 2.16 A resolution complexed with citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 111.926, 111.926, 102.094 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.074 - 2.160 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zvs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.290 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.100 | 0.880 |
| Number of reflections | 20756 | |
| <I/σ(I)> | 17.12 | 2.72 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 12.9 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.9 M AMMONIUM SULFATE, 0.1 M CITRIC ACID PH 4.0 |
