4A3V
yeast regulatory particle proteasome assembly chaperone Hsm3 in complex with Rpt1 C-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 186.660, 186.660, 373.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.350 - 3.800 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.20850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.910 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.080 | 0.710 |
| Number of reflections | 38668 | |
| <I/σ(I)> | 21.32 | 2.32 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 15.6 | 11.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 5M SODIUM FORMATE, 0.1M HEPES PH 7.5 |
