4A3T
yeast regulatory particle proteasome assembly chaperone Hsm3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.070, 94.950, 129.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.750 - 2.100 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.21940 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 62865 | |
| <I/σ(I)> | 22.99 | 5.18 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6.86 | 6.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20% PEG 4000, 0.2 M MALATE IMIDAZOLE, PH 5.5. |
