4A3S
Crystal structure of PFK from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.429, 77.007, 101.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.736 - 2.300 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.27550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pfk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.360 |
| Number of reflections | 35387 | |
| <I/σ(I)> | 11.9 | 3.4 |
| Completeness [%] | 97.4 | 84.2 |
| Redundancy | 5.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM NA HEPES PH 7.0, 12% (W/V) PEG 6000. |
