4A3D

RNA Polymerase II initial transcribing complex with a 6nt DNA-RNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	221.910, 391.360, 283.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.960 - 3.400
R-factor	0.1616
Rwork	0.161
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.330
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.400
Rmerge	0.930
Number of reflections	168619
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		pH 7