4A2W
Structure of full-length duck RIG-I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 163.320, 163.320, 152.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.770 - 3.700 |
| R-factor | 0.22118 |
| Rwork | 0.218 |
| R-free | 0.27365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RIG-I HELICASE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.000 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.100 | 0.860 |
| Number of reflections | 22592 | |
| <I/σ(I)> | 15.8 | 2.3 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 7.93 | 8.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 MG/ML PROTEIN 0.1 M KCL, 0.01M MGCL2, TRIS PH 8.5, 30% PEG 4000. |
