4A2V
Structure of duck RIG-I C-terminal domain (CTD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-22 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.320, 75.610, 38.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.120 - 1.440 |
| R-factor | 0.17429 |
| Rwork | 0.173 |
| R-free | 0.19352 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.230 |
| Number of reflections | 29376 | |
| <I/σ(I)> | 13.7 | 3.2 |
| Completeness [%] | 97.5 | 88.1 |
| Redundancy | 4.13 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25MG/ML WITH 0.1 M TRIS PH 8.6, 22% (W/V) PEG 6000 |
