4A2A
Thermotoga maritima FtsA:FtsZ(336-351)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.719, 75.718, 100.265 |
| Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
| Resolution | 47.980 - 1.800 |
| R-factor | 0.2127 |
| Rwork | 0.211 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e4f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.078 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.580 |
| Number of reflections | 74365 | |
| <I/σ(I)> | 10 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BISTRIS PH 5.5, 12 % (W/V) PEG 10K, 0.15 M AMMONIUM ACETATE. |
