4A27
Crystal structure of human synaptic vesicle membrane protein VAT-1 homolog-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.509, 62.104, 83.795 |
| Unit cell angles | 90.00, 106.98, 90.00 |
Refinement procedure
| Resolution | 19.590 - 2.100 |
| R-factor | 0.18702 |
| Rwork | 0.185 |
| R-free | 0.22956 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1yb5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 40044 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.20 M NABR, 0.1 M BISTRISPROPANE PH 6.5, 20.0% PEG 3350, 10.0% ETHYLENE GLYCOL. |
