4A25
X-ray structure Dps from Kineococcus radiotolerans in complex with Mn (II) ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 150.474, 150.474, 86.618 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.240 - 2.000 |
| R-factor | 0.19608 |
| Rwork | 0.194 |
| R-free | 0.23226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z90 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.151 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 49300 | |
| <I/σ(I)> | 40.94 | 7.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.7 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | MPD (2-METHYL-2,4-PENTANEDIOL) 45 %, TRIS-HCL 0.1 M, PH 8.0. |
