4A20
Crystal structure of the Ubl domain of Mdy2 (Get5) at 1.78A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 47.190, 47.190, 78.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.770 - 1.780 |
| R-factor | 0.20327 |
| Rwork | 0.201 |
| R-free | 0.24825 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m62 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.987 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.870 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 10128 | |
| <I/σ(I)> | 5.75 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.72 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 3 M AMMONIUM SULFATE, 100 MM SODIUM CITRATE PH 5.5 MIXED WITH AN EQUAL VOLUME OF PROTEIN AT 6MG/ML, HANGING DROP VAPOUR DIFFUSION. |
