4A1S
Crystallographic structure of the Pins:Insc complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-04-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.200, 64.232, 107.599 |
| Unit cell angles | 90.00, 117.90, 90.00 |
Refinement procedure
| Resolution | 23.132 - 2.100 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.25590 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.589 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.224 |
| Number of reflections | 56295 | |
| <I/σ(I)> | 12.5 | 2.3 |
| Completeness [%] | 98.7 | 96.7 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M MAGNESIUM CLORIDE, 15% PEG 4000, 0.1 M TRIS-HCL PH 8.5 |
