4A1N

Human Mitochondrial endo-exonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-21
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 31 2 1
Unit cell lengths	103.180, 103.180, 83.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.680 - 2.800
R-factor	0.1776
Rwork	0.175
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ism
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.160	0.690
Number of reflections	13052
<I/σ(I)>	13.8	3.8
Completeness [%]	100.0	100
Redundancy	11	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1