4A1F

Crystal structure of C-terminal domain of Helicobacter pylori DnaB Helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 2
Unit cell lengths	100.859, 102.164, 85.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.178 - 2.500
R-factor	0.219
Rwork	0.217
R-free	0.25110
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.005
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.590
Number of reflections	30696
<I/σ(I)>	16.2	2.5
Completeness [%]	99.0	99.8
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M CITRIC ACID PH 5.0, 2-10% W/V POLYETHYLENE GLYCOL 6000.