4A14
HUMAN KIF7, A KINESIN INVOLVED IN HEDGEHOG SIGNALLING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2010-07-19 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.991, 79.799, 95.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.459 - 1.600 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b6v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.547 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.500 | 1.600 |
| Rmerge | 0.030 | 0.430 |
| Number of reflections | 46998 | |
| <I/σ(I)> | 30.1 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% POLYETHYLENE GLYCOL 3350, 0.20 M LITHIUM ACETATE. |
