4A0Y
Structure of the global transcription regulator FapR from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.520, 110.520, 115.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.18651 |
| Rwork | 0.185 |
| R-free | 0.21124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0x |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.615 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 24146 | |
| <I/σ(I)> | 16.7 | 3.4 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 3.5 M NACL, 0.1 MM HEPES PH 7.5 |
