4A0X
Structure of the global transcription regulator FapR from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-16 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.580, 116.580, 123.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.090 - 2.400 |
| R-factor | 0.19867 |
| Rwork | 0.196 |
| R-free | 0.24617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f3x |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.638 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.630 |
| Number of reflections | 12145 | |
| <I/σ(I)> | 15.6 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 6% PEG-8000, 0.1M MES, 0.2 M ZN ACETATE, PH 5.4 |
