4A0U
Structure of the carboxy-terminal domain of bacteriophage T7 fibre gp17 containing residues 371-553, C2221 crystal form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.320, 145.560, 172.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.000 |
| R-factor | 0.15135 |
| Rwork | 0.149 |
| R-free | 0.19675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.370 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.340 |
| Number of reflections | 58236 | |
| <I/σ(I)> | 6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10 MM TRIS-HCL, 0.2 M AMMONIUM CITRATE (PH 7.0), 20 % (W/V) POLY-ETHYLENE GLYCOL 3350. |
