4A0R

Structure of bifunctional DAPA aminotransferase-DTB synthetase from Arabidopsis thaliana bound to dethiobiotin (DTB).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	C 1 2 1
Unit cell lengths	246.670, 76.630, 79.840
Unit cell angles	90.00, 108.02, 90.00

Refinement procedure

Resolution	40.436 - 2.680
R-factor	0.1881
Rwork	0.184
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE DETHIOBIOTIN SYNTHETASE- DIAMINOPELARGONIC ACID AMINOTRANSFERASE FROM ARABIDOPSIS THALIANA
RMSD bond length	0.009
RMSD bond angle	1.253
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.440	2.750
High resolution limit [Å]	2.680	2.680
Rmerge	0.150	0.760
Number of reflections	40026
<I/σ(I)>	9.95	1.83
Completeness [%]	99.7	99.8
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M AMMONIUM TARTRATE PH 6.2, 15 % PEG 3350.