4A0E
Crystal structure of the cytoplasmic N-terminal domain of Yersinia pestis YscD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 118.227, 118.227, 118.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.409 - 2.042 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.25580 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.060 | 0.640 |
| Number of reflections | 18490 | |
| <I/σ(I)> | 41.6 | 2.1 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 5.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.085M TRIS HCL PH 8.5, 25.5% (W/V) PEG 4000, 0.17M NA ACETATE, 15% (V/V) GLYCEROL, 10 MM TCEP HYDROCHLORIDE |
