4A09
Structure of hsDDB1-drDDB2 bound to a 15 bp CPD-duplex (purine at D-1 position) at 3.1 A resolution (CPD 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.650, 122.940, 154.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.270 - 3.100 |
| R-factor | 0.24833 |
| Rwork | 0.245 |
| R-free | 0.30753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ei1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.130 | 0.580 |
| Number of reflections | 39092 | |
| <I/σ(I)> | 16 | 4.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.3 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 100 MM MES, 15 MM NAOH, 21% PEG 200, pH 5.3 |
