483D
CRYSTAL STRUCTURE OF THE SARCIN/RICIN DOMAIN FROM E. COLI 23 S RRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-05-14 |
| Detector | ADSC |
| Spacegroup name | P 43 |
| Unit cell lengths | 29.481, 29.481, 76.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.110 |
| R-factor | 0.152 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THE RELATED RAT SARCIN/RICIN STRUCTURE (430D) |
| RMSD bond length | 0.029 * |
| RMSD bond angle | 0.035 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.120 |
| High resolution limit [Å] | 1.110 | 1.110 |
| Rmerge | 0.042 | 0.487 |
| Number of reflections | 25407 | |
| <I/σ(I)> | 35.4 | 2.2 |
| Completeness [%] | 97.9 | 84.5 * |
| Redundancy | 4.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 19 * | 3.0 - 3.2 M (NH4)2SO4, (50 MM KMOPS, 10 MM MGCL2, 10MM MNCL2), 2.5 MG/ML RNA, pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KMOPS | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | MNCL2 | ||
| 4 | 1 | 1 | (NH4)2SO4 | ||
| 5 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | Na-EDTA | 1.0 (mM) | |
| 3 | 1 | drop | Tris | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.0-3.2 (M) | |
| 5 | 1 | reservoir | potassium MOPS | 50 (mM) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | 10 (mM) |
