472D
STRUCTURE OF AN OCTAMER RNA WITH TANDEM GG/UU MISPAIRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-16 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 |
| Unit cell lengths | 41.920, 41.920, 56.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.055 * |
| Number of reflections | 2708 |
| Completeness [%] | 98.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 2 MM RNA (SINGLE STRAND), 0.04 M NA CACODYLATE (PH 7.0), 5% MPD, 0.050 M MGCL2, 0.001 M COBALT HEXAMINE CHORIDE, 55% MPD IN RESERVOIR, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CACODYLATE | ||
| 2 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | cobalt hexamine chloride | 1.0 (mM) | |
| 4 | 1 | drop | 5 (%(v/v)) | ||
| 5 | 1 | reservoir | 55 (%) |
