467D
The structure of a decamer forming a four-way junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.200, 23.740, 38.300 |
| Unit cell angles | 90.00, 112.43, 90.00 |
Refinement procedure
| Resolution | 19.390 - 2.160 |
| R-factor | 0.257 |
| Rwork | 0.240 |
| R-free | 0.28800 |
| Structure solution method | MAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.390 | 2.230 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.069 | 0.137 |
| Number of reflections | 2786 | |
| <I/σ(I)> | 10.4 | 5.2 |
| Completeness [%] | 92.0 | 75.5 |
| Redundancy | 2.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 6.7% MPD, 0.2M MGCL2, 1.0MM DNA DECAMER AGAINST 45% MPD IN DEPOSIT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | MPD | 10 (%) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | reservoir | MPD | 45 (%) | 0.5ml |
