464D
DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.375, 28.995, 28.995 |
| Unit cell angles | 110.37, 96.62, 96.62 |
Refinement procedure
| Resolution | 15.000 - 1.230 |
| R-factor | 0.127 * |
| Rwork | 0.127 |
| R-free | 0.19330 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 0.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.240 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.050 | 0.308 |
| Total number of observations | 64180 * | |
| Number of reflections | 18837 * | |
| Completeness [%] | 82.8 | 32.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.5 MM RNA DUPLEX, 12 MM SPERMINE, 40 MM NA-CACODYLATE PH 6.0, 80 MM SRCL2,40 MM LICL, 10 % (V/V) MPD AGAINST 0.5 ML 35 % (V/V) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | SRCL2 | ||
| 4 | 1 | 1 | LICL | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | reservoir | sodium cacodylate | 40 (mM) | |
| 3 | 1 | reservoir | spermine tetra-HCl | 12 (mM) | |
| 4 | 1 | reservoir | 80 (mM) | ||
| 5 | 1 | reservoir | 40 (mM) | ||
| 6 | 1 | reservoir | 10 (%(v/v)) |
