463D
CALCIUM FORM OF D(CGCGAATTCGCG)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-10 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 41.117, 41.117, 99.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.450 |
| R-factor | 0.2172 |
| R-free | 0.25680 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.510 | |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.053 | 0.337 |
| Number of reflections | 11038 | |
| Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | CRYSTALLIZED FROM SOLUTION OF OLIGONUCLEOTIDE, CACL2, MPD, AND CACODYLIC ACID IN A HANGING DROP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CACL2 | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACODYLIC ACID | ||
| 4 | 1 | 2 | CACL2 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | CACODYLIC ACID |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dodecamer | 0.4 (mM) | |
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | MPD | 10 (%) | |
| 4 | 1 | drop | cacodylic acid | 20 (mM) | |
| 5 | 1 | reservoir | 48 (%) |
