45AZ

Crystal structure of the emerging Songling virus nucleoprotein

Replaces:  9X6Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 2 21 21
Unit cell lengths	54.530, 113.480, 215.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.560 - 2.000
R-factor	0.1944
Rwork	0.194
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.912
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.560	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.159	4.000
Number of reflections	91608	1997
<I/σ(I)>	11.9	1.23
Completeness [%]	99.9	100
Redundancy	15
CC(1/2)		0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	90 mM LiNaK (0.3 M Lithium sulfate, 0.3 M Sodium sulfate, 0.3 M Potassium sulfate); 0.1 M Buffer System 4 (1.0M MOPSO, Bis-Tris, PH 6.5); PH 6.5; 50 % v/v Precipitant Mix 6 (25% w/v PEG 4000, 40% w/v 1,2,6- Hexanetriol)