435D
5'-R(*UP*AP*GP*CP*CP*CP*C)-3', 5'-R(*GP*GP*GP*GP*CP*UP*A)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.680, 26.697, 30.459 |
| Unit cell angles | 104.29, 104.22, 91.66 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| Rwork | 0.159 |
| R-free | 0.21800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CANONICAL A-FORM RNA DUPLEX |
| RMSD bond length | 0.028 |
| RMSD bond angle | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.057 | 0.288 |
| Number of reflections | 13834 | |
| Completeness [%] | 89.5 * | 80.7 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 21 * | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 2 | 1 | drop | spermine tetra-HCl | 12 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 10 (%(v/v)) | ||
| 5 | 1 | drop | RNA | 0.5 (mM) | |
| 6 | 1 | reservoir | MPD | 35 (%(v/v)) |
