435D

5'-R(*UP*AP*GP*CP*CP*CP*C)-3', 5'-R(*GP*GP*GP*GP*CP*UP*A)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11-28
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	26.680, 26.697, 30.459
Unit cell angles	104.29, 104.22, 91.66

Refinement procedure

Resolution	15.000 - 1.400
Rwork	0.159
R-free	0.21800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CANONICAL A-FORM RNA DUPLEX
RMSD bond length	0.028
RMSD bond angle	0.010
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.057	0.288
Number of reflections	13834
Completeness [%]	89.5 *	80.7
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	21 *	pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SPERMINE
2	1	1	KCL
3	1	1	MPD
4	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium cacodylate	40 (mM)
2	1	drop	spermine tetra-HCl	12 (mM)
3	1	drop		100 (mM)
4	1	drop		10 (%(v/v))
5	1	drop	RNA	0.5 (mM)
6	1	reservoir	MPD	35 (%(v/v))